If producing v2 sample sheets for devices apart from those listed, validate the ultimate sample sheet has the i5 index in the proper orientation as described above.
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If jogging in handbook mode and employing bcl2fastq or BCL Transform, or uploading the sample sheet to BaseSpace to be a handbook manner operate and attaching a sample sheet, enter the i5 sequence within the ahead direction.
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Therefore these software program work on the assumption that your i5 sequence is always inside the forward orientation and can routinely make the reverse enhance of your sequences provided for the appropriate instrument kind.
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